› 1a0f

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF GLUTATHIONE S-TRANSFERASE FROM ESCHERICHIA COLI COMPLEXED WITH GLUTATHIONESULFONIC ACID

Released:
DOI: 10.2210/pdb1a0f/pdb
PDB entry 1a0f coloured by chain, front view.
PDB entry 1a0f coloured by chain, side view.
PDB entry 1a0f coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Three-dimensional structure of Escherichia coli glutathione S-transferase complexed with glutathione sulfonate: catalytic roles of Cys10 and His106.
Nishida M, Harada S, Noguchi S, Satow Y, Inoue H, Takahashi K
J Mol Biol 281 135-47 (1998)
PMID: 9680481

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase GstA Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.9 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A9D2 (Residues: 1-201; Coverage: 100%)
Gene names: JW1627, b1635, gst, gstA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTS 2 x GTS
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 90.65Å b: 95.39Å c: 51.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.245
Expression system: Escherichia coli K-12

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Citations

3 review citations

Glutathione transferases in bacteria.
Allocati et al. (2009)
2 more

12 mentions without citation

FINDSITE: a combined evolution/structure-based approach to protein function prediction.
Skolnick et al. (2009)
11 more