› 1a0i

X-ray diffraction
2.6Å resolution

ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7 COMPLEX WITH ATP

Released:
DOI: 10.2210/pdb1a0i/pdb
PDB entry 1a0i coloured by chain, front view.
PDB entry 1a0i coloured by chain, side view.
PDB entry 1a0i coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7.
Subramanya HS, Doherty AJ, Ashford SR, Wigley DB
Cell 85 607-15 (1996)
PMID: 8653795

Function and Biology Details

Reaction catalysed: 
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.89 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00969 (Residues: 2-349; Coverage: 97%)
Gene name: 1.3
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P21212
Unit cell:
a: 64.7Å b: 85.2Å c: 79.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.341
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Base flipping.
Roberts et al. (1998)
23 more

27 mentions without citation

Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives.
Mueser et al. (2010)
26 more