› 1a2e

X-ray diffraction
1.63Å resolution

CRYSTAL STRUCTURE OF A DOUBLE-STRANDED DNA DECAMER CONTAINING A CISPLATIN INTERSTRAND CROSS-LINK ADDUCT

Released:
DOI: 10.2210/pdb1a2e/pdb
PDB entry 1a2e coloured by chain, front view.
PDB entry 1a2e coloured by chain, side view.
PDB entry 1a2e coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure of a double-stranded DNA containing a cisplatin interstrand cross-link at 1.63 A resolution: hydration at the platinated site.
Coste F, Malinge JM, Serre L, Shepard W, Roth M, Leng M, Zelwer C
Nucleic Acids Res 27 1837-46 (1999)
PMID: 10101191

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113388 (preferred)
Entry contents:
2 distinct DNA molecules
Macromolecules (2 distinct):
DNA (5'-D(*CP*CP*TP*CP*GP*CP*TP*CP*TP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 2.93 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*AP*GP*AP*GP*CP*GP*AP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.16 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CPT 1 x CPT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW21B
Spacegroup: C2
Unit cell:
a: 42.89Å b: 29.98Å c: 46.57Å
α: 90° β: 95.98° γ: 90°
R-values:
R R work R free
0.169 not available not available
Expression system: Not provided

Quick links

Citations

13 review citations

Formation and repair of interstrand cross-links in DNA.
Noll et al. (2006)
12 more

3 mentions without citation

The Next Generation of Platinum Drugs: Targeted Pt(II) Agents, Nanoparticle Delivery, and Pt(IV) Prodrugs.
Johnstone et al. (2016)
2 more