› 1a32

X-ray diffraction
2.1Å resolution

RIBOSOMAL PROTEIN S15 FROM BACILLUS STEAROTHERMOPHILUS

Released:
DOI: 10.2210/pdb1a32/pdb
PDB entry 1a32 coloured by chain, front view.
PDB entry 1a32 coloured by chain, side view.
PDB entry 1a32 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Conformational variability of the N-terminal helix in the structure of ribosomal protein S15.
Clemons WM, Davies C, White SW, Ramakrishnan V
Structure 6 429-38 (1998)
PMID: 9562554

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small ribosomal subunit protein uS15 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.59 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05766 (Residues: 2-89; Coverage: 99%)
Gene name: rpsO
Sequence domains: Ribosomal protein S15
Structure domains: S15/NS1, RNA-binding

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 33.02Å b: 33.75Å c: 75.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.318
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Structural insights into the signal recognition particle.
Doudna et al. (2004)
6 more

20 mentions without citation

An accurate, residue-level, pair potential of mean force for folding and binding based on the distance-scaled, ideal-gas reference state.
Zhang et al. (2004)
19 more