› 1a4m

X-ray diffraction
1.95Å resolution

ADA STRUCTURE COMPLEXED WITH PURINE RIBOSIDE AT PH 7.0

Released:
DOI: 10.2210/pdb1a4m/pdb
PDB entry 1a4m coloured by chain, front view.
PDB entry 1a4m coloured by chain, side view.
PDB entry 1a4m coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Complexes of adenosine deaminase with two potent inhibitors: X-ray structures in four independent molecules at pH of maximum activity.
Wang Z, Quiocho FA
Biochemistry 37 8314-24 (1998)
PMID: 9622483

Function and Biology Details

Reaction catalysed: 
2'-deoxyadenosine + H(2)O = 2'-deoxyinosine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine deaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 349 amino acids
Theoretical weight: 39.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03958 (Residues: 4-352; Coverage: 99%)
Gene name: Ada
Sequence domains: Adenosine deaminase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand PRH 4 x PRH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 88.693Å b: 93.644Å c: 102.177Å
α: 90° β: 102.84° γ: 90°
R-values:
R R work R free
0.193 0.193 0.281
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Divergent evolution of enzymatic function: mechanistically diverse superfamilies and functionally distinct suprafamilies.
Gerlt et al. (2001)
4 more

10 mentions without citation

Structural bases for CRMP function in plexin-dependent semaphorin3A signaling.
Deo et al. (2004)
9 more