› 1a5t

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III

Released:
DOI: 10.2210/pdb1a5t/pdb
PDB entry 1a5t coloured by chain, front view.
PDB entry 1a5t coloured by chain, side view.
PDB entry 1a5t coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III.
Guenther B, Onrust R, Sali A, O'Donnell M, Kuriyan J
Cell 91 335-45 (1997)
PMID: 9363942

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit delta' Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 36.98 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P28631 (Residues: 1-334; Coverage: 100%)
Gene names: JW1085, b1099, holB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 98.9Å b: 104Å c: 75.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.265
Expression system: Escherichia coli

Quick links

Citations

48 review citations

Comparative protein structure modeling of genes and genomes.
Martí-Renom et al. (2000)
47 more

12 mentions without citation

Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).
Zhao et al. (2012)
11 more