› 1a62

X-ray diffraction
1.55Å resolution

CRYSTAL STRUCTURE OF THE RNA-BINDING DOMAIN OF THE TRANSCRIPTIONAL TERMINATOR PROTEIN RHO

Released:
DOI: 10.2210/pdb1a62/pdb
PDB entry 1a62 coloured by chain, front view.
PDB entry 1a62 coloured by chain, side view.
PDB entry 1a62 coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Crystal structure of the RNA-binding domain from transcription termination factor rho.
Allison TJ, Wood TC, Briercheck DM, Rastinejad F, Richardson JP, Rule GS
Nat Struct Biol 5 352-6 (1998)
PMID: 9586995

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination factor Rho Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.78 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG30 (Residues: 1-130; Coverage: 31%)
Gene names: JW3756, b3783, nitA, psuA, rho, rnsC, sbaA, tsu
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 25.95Å b: 56.01Å c: 42.92Å
α: 90° β: 99.35° γ: 90°
R-values:
R R work R free
0.216 0.216 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Nucleic acid recognition by OB-fold proteins.
Theobald et al. (2003)
5 more

7 mentions without citation

Evaluating conformational free energies: the colony energy and its application to the problem of loop prediction.
Xiang et al. (2002)
6 more