› 1a97

X-ray diffraction
2.6Å resolution

XPRTASE FROM E. COLI COMPLEXED WITH GMP

Released:
DOI: 10.2210/pdb1a97/pdb
Model geometry
Fit model/data
PDB entry 1a97 coloured by chain, front view.
PDB entry 1a97 coloured by chain, side view.
PDB entry 1a97 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures of free and complexed forms of Escherichia coli xanthine-guanine phosphoribosyltransferase.
Vos S, Parry RJ, Burns MR, de Jersey J, Martin JL
J Mol Biol 282 875-89 (1998)
PMID: 9743633

Function and Biology Details

Reaction catalysed: 
XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xanthine-guanine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 148 amino acids
Theoretical weight: 16.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9M5 (Residues: 3-150; Coverage: 97%)
Gene names: JW0228, b0238, gpp, gpt, gxu
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand BO3 4 x BO3
Ligand 5GP 2 x 5GP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RUH2R
Spacegroup: P43212
Unit cell:
a: 94.042Å b: 94.042Å c: 166.914Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.263
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Direct binding targets of the stringent response alarmone (p)ppGpp.
Kanjee et al. (2012)
1 more

3 mentions without citation

The good, the bad and the dubious: VHELIBS, a validation helper for ligands and binding sites.
Cereto-Massagué et al. (2013)
2 more