› 1a98

X-ray diffraction
2.25Å resolution

XPRTASE FROM E. COLI COMPLEXED WITH GMP

Released:
DOI: 10.2210/pdb1a98/pdb
PDB entry 1a98 coloured by chain, front view.
PDB entry 1a98 coloured by chain, side view.
PDB entry 1a98 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures of free and complexed forms of Escherichia coli xanthine-guanine phosphoribosyltransferase.
Vos S, Parry RJ, Burns MR, de Jersey J, Martin JL
J Mol Biol 282 875-89 (1998)
PMID: 9743633

Function and Biology Details

Reaction catalysed: 
XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xanthine-guanine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 16.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9M5 (Residues: 1-152; Coverage: 100%)
Gene names: JW0228, b0238, gpp, gpt, gxu
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 84.166Å b: 70.927Å c: 54.051Å
α: 90° β: 113.4° γ: 90°
R-values:
R R work R free
0.21 0.21 0.224
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Direct binding targets of the stringent response alarmone (p)ppGpp.
Kanjee et al. (2012)
1 more