› 1a9t

X-ray diffraction
2Å resolution

BOVINE PURINE NUCLEOSIDE PHOSPHORYLASE COMPLEXED WITH 9-DEAZAINOSINE AND PHOSPHATE

Released:
DOI: 10.2210/pdb1a9t/pdb
PDB entry 1a9t coloured by chain, front view.
PDB entry 1a9t coloured by chain, side view.
PDB entry 1a9t coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues.
Mao C, Cook WJ, Zhou M, Federov AA, Almo SC, Ealick SE
Biochemistry 37 7135-46 (1998)
PMID: 9585525

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 31.57 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P55859 (Residues: 1-284; Coverage: 98%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand R1P 1 x R1P
Ligand HPA 1 x HPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P213
Unit cell:
a: 94.2Å b: 94.2Å c: 94.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 not available

Quick links

Citations

8 review citations

Purine nucleoside phosphorylases: properties, functions, and clinical aspects.
Bzowska et al. (2000)
7 more

6 mentions without citation

Atomic detail of chemical transformation at the transition state of an enzymatic reaction.
Saen-Oon et al. (2008)
5 more