› 1ad2

X-ray diffraction
1.9Å resolution

RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE

Released:
DOI: 10.2210/pdb1ad2/pdb
PDB entry 1ad2 coloured by chain, front view.
PDB entry 1ad2 coloured by chain, side view.
PDB entry 1ad2 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
A mutant form of the ribosomal protein L1 reveals conformational flexibility.
Unge J, Al-Karadaghi S, Liljas A, Jonsson BH, Eliseikina I, Ossina N, Nevskaya N, Fomenkova N, Garber M, Nikonov S
FEBS Lett 411 53-9 (1997)
PMID: 9247141

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein uL1 Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 24.75 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P27150 (Residues: 2-229; Coverage: 100%)
Gene names: rpl1, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand HG 1 x HG
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21212
Unit cell:
a: 76.1Å b: 61.4Å c: 46.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.27
Expression system: Escherichia coli BL21

Quick links

Citations

6 citation in other articles

A protein-RNA docking benchmark (I): nonredundant cases.
Barik et al. (2012)
5 more

4 mentions without citation

Phosphorylated Lamin A/C in the Nuclear Interior Binds Active Enhancers Associated with Abnormal Transcription in Progeria.
Ikegami et al. (2020)
3 more