› 1add

X-ray diffraction
2.4Å resolution

A PRE-TRANSITION STATE MIMIC OF AN ENZYME: X-RAY STRUCTURE OF ADENOSINE DEAMINASE WITH BOUND 1-DEAZA-ADENOSINE AND ZINC-ACTIVATED WATER

Released:
DOI: 10.2210/pdb1add/pdb
PDB entry 1add coloured by chain, front view.
PDB entry 1add coloured by chain, side view.
PDB entry 1add coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A pre-transition-state mimic of an enzyme: X-ray structure of adenosine deaminase with bound 1-deazaadenosine and zinc-activated water.
Wilson DK, Quiocho FA
Biochemistry 32 1689-94 (1993)
PMID: 8439534

Function and Biology Details

Reaction catalysed: 
2'-deoxyadenosine + H(2)O = 2'-deoxyinosine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine deaminase Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03958 (Residues: 4-352; Coverage: 99%)
Gene name: Ada
Sequence domains: Adenosine deaminase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand 1DA 1 x 1DA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 102.39Å b: 94.28Å c: 73.02Å
α: 90° β: 127.05° γ: 90°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Divergent evolution of enzymatic function: mechanistically diverse superfamilies and functionally distinct suprafamilies.
Gerlt et al. (2001)
9 more

20 mentions without citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
19 more