1ae1

X-ray diffraction
2.4Å resolution

TROPINONE REDUCTASE-I COMPLEX WITH NADP

Released:

Function and Biology Details

Reaction catalysed:
Tropine + NADP(+) = tropinone + NADPH
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-156136 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tropinone reductase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 29.65 KDa
Source organism: Datura stramonium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50162 (Residues: 1-273; Coverage: 100%)
Gene name: TR1
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 55.74Å b: 122.75Å c: 75.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.155 0.248
Expression system: Escherichia coli BL21(DE3)