› 1af9

X-ray diffraction
2.7Å resolution

TETANUS NEUROTOXIN C FRAGMENT

Released:
DOI: 10.2210/pdb1af9/pdb
PDB entry 1af9 coloured by chain, front view.
PDB entry 1af9 coloured by chain, side view.
PDB entry 1af9 coloured by chain, top view.
Source organism: Clostridium tetani
Primary publication:
Structure of the receptor binding fragment HC of tetanus neurotoxin.
Umland TC, Wingert LM, Swaminathan S, Furey WF, Schmidt JJ, Sax M
Nat Struct Biol 4 788-92 (1997)
PMID: 9334741

Function and Biology Details

Reaction catalysed: 
Hydrolysis of -Gln(76)-|-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetanus toxin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 452 amino acids
Theoretical weight: 51.83 KDa
Source organism: Clostridium tetani
Expression system: Escherichia coli
UniProt:
  • Canonical: P04958 (Residues: 865-1315; Coverage: 34%)
Gene names: CTC_p60, tetX
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 67.4Å b: 79.7Å c: 91.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.245
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Neurotoxins affecting neuroexocytosis.
Schiavo et al. (2000)
23 more

6 mentions without citation

Tetanus Toxin Fragment C: Structure, Drug Discovery Research and Production.
Bayart et al. (2022)
5 more