› 1ag9

X-ray diffraction
1.8Å resolution

FLAVODOXINS THAT ARE REQUIRED FOR ENZYME ACTIVATION: THE STRUCTURE OF OXIDIZED FLAVODOXIN FROM ESCHERICHIA COLI AT 1.8 ANGSTROMS RESOLUTION.

Released:
DOI: 10.2210/pdb1ag9/pdb
PDB entry 1ag9 coloured by chain, front view.
PDB entry 1ag9 coloured by chain, side view.
PDB entry 1ag9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A flavodoxin that is required for enzyme activation: the structure of oxidized flavodoxin from Escherichia coli at 1.8 A resolution.
Hoover DM, Ludwig ML
Protein Sci 6 2525-37 (1997)
PMID: 9416602

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P61949 (Residues: 2-176; Coverage: 99%)
Gene names: JW0671, b0684, fldA
Sequence domains: Flavodoxin
Structure domains: Rossmann fold

Ligands and Environments

5 bound ligands:
Ligand CA 4 x CA
Ligand CL 2 x CL
Ligand NA 4 x NA
Ligand FMN 2 x FMN
Ligand BTB 2 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 126.4Å b: 41.1Å c: 68.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily.
Munro et al. (2007)
2 more

5 mentions without citation

A Structural-informatics approach for tracing beta-sheets: building pseudo-C(alpha) traces for beta-strands in intermediate-resolution density maps.
Kong et al. (2004)
4 more