› 1agu

Solution NMR

THE SOLUTION NMR STRUCTURE OF THE C10R ADDUCT OF BENZO[A]PYRENE-DIOL-EPOXIDE AT THE N6 POSITION OF ADENINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 60-62 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1agu/pdb
PDB entry 1agu coloured by chain, front view.
PDB entry 1agu coloured by chain, side view.
PDB entry 1agu coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Adduction of the human N-ras codon 61 sequence with (-)-(7S,8R,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene: structural refinement of the intercalated SRSR(61,2) (-)-(7S,8R,9S,10R)-N6-[10-(7,8,9,10- tetrahydrobenzo[a]pyrenyl)]-2'-deoxyadenosyl adduct from 1H NMR.
Zegar IS, Kim SJ, Johansen TN, Horton PJ, Harris CM, Harris TM, Stone MP
Biochemistry 35 6212-24 (1996)
PMID: 8639561

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113420 (preferred)
Entry contents:
2 distinct DNA molecules
Macromolecules (2 distinct):
DNA (5'-D(*CP*GP*GP*AP*CP*EP*AP*GP*AP*AP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 11 nucleotides
Theoretical weight: 3.72 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*TP*TP*CP*TP*TP*GP*TP*CP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand E 1 x E

Experiments and Validation Details

Entry percentile scores
Refinement method: NOE-RESTRAINED MOLECULAR DYNAMICS/SIMULATED ANNEALING
Expression system: Not provided

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Citations

5 review citations

Oxysterols: modulators of cholesterol metabolism and other processes.
Schroepfer GJ. (2000)
4 more

1 mention without citation

Stereochemistry and position-dependent effects of carcinogens on TATA/TBP binding.
Zhang et al. (2006)