› 1ahq

X-ray diffraction
2.3Å resolution

RECOMBINANT ACTOPHORIN

Released:
DOI: 10.2210/pdb1ahq/pdb
PDB entry 1ahq coloured by chain, front view.
PDB entry 1ahq coloured by chain, side view.
PDB entry 1ahq coloured by chain, top view.
Source organism: Acanthamoeba castellanii
Primary publication:
Crystal structure of the actin-binding protein actophorin from Acanthamoeba.
Leonard SA, Gittis AG, Petrella EC, Pollard TD, Lattman EE
Nat Struct Biol 4 369-73 (1997)
PMID: 9145107

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actophorin Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.44 KDa
Source organism: Acanthamoeba castellanii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37167 (Residues: 2-138; Coverage: 99%)
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 39.295Å b: 47.486Å c: 67.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.32
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Proteins of the ADF/cofilin family: essential regulators of actin dynamics.
Bamburg JR. (1999)
8 more

10 mentions without citation

The ADF/cofilin family: actin-remodeling proteins.
Maciver et al. (2002)
9 more