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Solution NMR

NMR STRUCTURES OF THE CELLULOSE-BINDING DOMAIN OF THE ENDOGLUCANASE Z FROM ERWINIA CHRYSANTHEMI, 23 STRUCTURES

Released:
DOI: 10.2210/pdb1aiw/pdb
PDB entry 1aiw coloured by chain, ensemble of 23 models, front view.
PDB entry 1aiw coloured by chain, ensemble of 23 models, side view.
PDB entry 1aiw coloured by chain, ensemble of 23 models, top view.
Source organism: Dickeya dadantii 3937
Primary publication:
Solution structure of the cellulose-binding domain of the endoglucanase Z secreted by Erwinia chrysanthemi.
Brun E, Moriaud F, Gans P, Blackledge MJ, Barras F, Marion D
Biochemistry 36 16074-86 (1997)
PMID: 9405041

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Z Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 6.66 KDa
Source organism: Dickeya dadantii 3937
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07103 (Residues: 366-426; Coverage: 16%)
Gene names: Dda3937_02793, cel5, cel5Z, celZ
Sequence domains: Cellulose-binding domain
Structure domains: Carbohydrate-binding module superfamily 5/12

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Cellulose, cellulases and cellulosomes.
Bayer et al. (1998)
3 more

9 mentions without citation

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
8 more