› 1ajr

X-ray diffraction
1.74Å resolution

REFINEMENT AND COMPARISON OF THE CRYSTAL STRUCTURES OF PIG CYTOSOLIC ASPARTATE AMINOTRANSFERASE AND ITS COMPLEX WITH 2-METHYLASPARTATE

Released:
DOI: 10.2210/pdb1ajr/pdb
PDB entry 1ajr coloured by chain, front view.
PDB entry 1ajr coloured by chain, side view.
PDB entry 1ajr coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate.
Rhee S, Silva MM, Hyde CC, Rogers PH, Metzler CM, Metzler DE, Arnone A
J Biol Chem 272 17293-302 (1997)
PMID: 9211866

Function and Biology Details

Reactions catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 412 amino acids
Theoretical weight: 46.62 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00503 (Residues: 2-413; Coverage: 100%)
Gene name: GOT1
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 125Å b: 130.8Å c: 55.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 not available

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Citations

10 review citations

Mechanical processes in biochemistry.
Bustamante et al. (2004)
9 more

6 mentions without citation

Sequence-structure analysis of FAD-containing proteins.
Dym et al. (2001)
5 more