› 1al3

X-ray diffraction
1.8Å resolution

COFACTOR BINDING FRAGMENT OF CYSB FROM KLEBSIELLA AEROGENES

Released:
DOI: 10.2210/pdb1al3/pdb
PDB entry 1al3 coloured by chain, front view.
PDB entry 1al3 coloured by chain, side view.
PDB entry 1al3 coloured by chain, top view.
Source organism: Klebsiella aerogenes
Primary publication:
The structure of the cofactor-binding fragment of the LysR family member, CysB: a familiar fold with a surprising subunit arrangement.
Tyrrell R, Verschueren KH, Dodson EJ, Murshudov GN, Addy C, Wilkinson AJ
Structure 5 1017-32 (1997)
PMID: 9309218

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator CysB Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.01 KDa
Source organism: Klebsiella aerogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P45600 (Residues: 1-324; Coverage: 100%)
Gene name: cysB
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 66.38Å b: 107.53Å c: 32.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.246
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
7 more

8 mentions without citation

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
7 more