› 1aln

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF CYTIDINE DEAMINASE COMPLEXED WITH 3-DEAZACYTIDINE

Released:
DOI: 10.2210/pdb1aln/pdb
PDB entry 1aln coloured by chain, front view.
PDB entry 1aln coloured by chain, side view.
PDB entry 1aln coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cytidine deaminase complexed to 3-deazacytidine: a "valence buffer" in zinc enzyme catalysis.
Xiang S, Short SA, Wolfenden R, Carter CW
Biochemistry 35 1335-41 (1996)
PMID: 8634261

Function and Biology Details

Reaction catalysed: 
2'-deoxycytidine + H(2)O = 2'-deoxyuridine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytidine deaminase Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 31.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABF6 (Residues: 1-294; Coverage: 100%)
Gene names: JW2131, b2143, cdd
Sequence domains:
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CTD 1 x CTD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3121
Unit cell:
a: 120.3Å b: 120.3Å c: 78.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 not available
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Enzymatic conversion of adenosine to inosine and to N1-methylinosine in transfer RNAs: a review.
Grosjean et al. (1996)
1 more

4 mentions without citation

Frequencies of amino acid strings in globular protein sequences indicate suppression of blocks of consecutive hydrophobic residues.
Schwartz et al. (2001)
3 more