› 1am2

X-ray diffraction
2.2Å resolution

GYRA INTEIN FROM MYCOBACTERIUM XENOPI

Released:
DOI: 10.2210/pdb1am2/pdb
PDB entry 1am2 coloured by chain, front view.
PDB entry 1am2 coloured by chain, side view.
PDB entry 1am2 coloured by chain, top view.
Source organism: Mycobacterium xenopi
Primary publication:
Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing.
Klabunde T, Sharma S, Telenti A, Jacobs WR, Sacchettini JC
Nat Struct Biol 5 31-6 (1998)
PMID: 9437427

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mxe GyrA intein Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.36 KDa
Source organism: Mycobacterium xenopi
Expression system: Escherichia coli
UniProt:
  • Canonical: P72065 (Residues: 67-263; Coverage: 60%)
Gene name: gyrA
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3221
Unit cell:
a: 55.7Å b: 55.7Å c: 133.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.187 0.252
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Semisynthesis of proteins by expressed protein ligation.
Muir TW. (2003)
24 more

11 mentions without citation

Chemical strategies to understand the language of ubiquitin signaling.
Weller et al. (2014)
10 more