› 1an9

X-ray diffraction
2.5Å resolution

D-AMINO ACID OXIDASE COMPLEX WITH O-AMINOBENZOATE

Released:
DOI: 10.2210/pdb1an9/pdb
PDB entry 1an9 coloured by chain, front view.
PDB entry 1an9 coloured by chain, side view.
PDB entry 1an9 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural and mechanistic studies on D-amino acid oxidase x substrate complex: implications of the crystal structure of enzyme x substrate analog complex.
Miura R, Setoyama C, Nishina Y, Shiga K, Mizutani H, Miyahara I, Hirotsu K
J Biochem 122 825-33 (1997)
PMID: 9399588

Function and Biology Details

Reaction catalysed: 
A D-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-amino-acid oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 38.61 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00371 (Residues: 1-340; Coverage: 98%)
Gene name: DAO
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand BE2 2 x BE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 109.31Å b: 92.58Å c: 71.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.26
Expression system: Escherichia coli

Quick links

Citations

4 review citations

D-Amino acid oxidase: structure, catalytic mechanism, and practical application.
Tishkov et al. (2005)
3 more

4 mentions without citation

Crystal structure of human D-amino acid oxidase: context-dependent variability of the backbone conformation of the VAAGL hydrophobic stretch located at the si-face of the flavin ring.
Kawazoe et al. (2006)
3 more