› 1aq0

X-ray diffraction
2Å resolution

BARLEY 1,3-1,4-BETA-GLUCANASE IN MONOCLINIC SPACE GROUP

Released:
DOI: 10.2210/pdb1aq0/pdb
PDB entry 1aq0 coloured by chain, front view.
PDB entry 1aq0 coloured by chain, side view.
PDB entry 1aq0 coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Crystal structure of barley 1,3-1,4-beta-glucanase at 2.0-A resolution and comparison with Bacillus 1,3-1,4-beta-glucanase.
Müller JJ, Thomsen KK, Heinemann U
J Biol Chem 273 3438-46 (1998)
PMID: 9452466

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lichenase-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 32.14 KDa
Source organism: Hordeum vulgare
UniProt:
  • Canonical: P12257 (Residues: 7-312; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 17
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P21
Unit cell:
a: 49.58Å b: 82.99Å c: 77.56Å
α: 90° β: 104.36° γ: 90°
R-values:
R R work R free
0.17 0.17 0.213

Quick links

Citations

1 review citation

Mapping of IgE-binding epitopes on the major latex allergen Hev b 2 and the cross-reacting 1,3beta-glucanase fruit allergens as a molecular basis for the latex-fruit syndrome.
Barre et al. (2009)
13 other articles

7 mentions without citation

Selection on Glycine beta-1,3-endoglucanase genes differentially inhibited by a Phytophthora glucanase inhibitor protein.
Bishop et al. (2005)
6 more