› 1aqj

X-ray diffraction
2.6Å resolution

STRUCTURE OF ADENINE-N6-DNA-METHYLTRANSFERASE TAQI

Released:
DOI: 10.2210/pdb1aqj/pdb
PDB entry 1aqj coloured by chain, front view.
PDB entry 1aqj coloured by chain, side view.
PDB entry 1aqj coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Differential binding of S-adenosylmethionine S-adenosylhomocysteine and Sinefungin to the adenine-specific DNA methyltransferase M.TaqI.
Schluckebier G, Kozak M, Bleimling N, Weinhold E, Saenger W
J Mol Biol 265 56-67 (1997)
PMID: 8995524

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II methyltransferase M.TaqI Chains: A, B
Molecule details ›
Chains: A, B
Length: 421 amino acids
Theoretical weight: 47.92 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14385 (Residues: 1-421; Coverage: 100%)
Gene name: taqIM
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SFG 2 x SFG
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P21212
Unit cell:
a: 130.45Å b: 142.94Å c: 53.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.286
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases.
Jeltsch A. (2002)
6 more

2 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
1 more