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› 1ar7

X-ray diffraction
2.9Å resolution

P1/MAHONEY POLIOVIRUS, DOUBLE MUTANT P1095S + H2142Y

Released:
DOI: 10.2210/pdb1ar7/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1ar7 coloured by chain, front view.
PDB entry 1ar7 coloured by chain, side view.
PDB entry 1ar7 coloured by chain, top view.
Source organism: Human poliovirus 1 Mahoney
Primary publication:
Structural studies of poliovirus mutants that overcome receptor defects.
Wien MW, Curry S, Filman DJ, Hogle JM
Nat Struct Biol 4 666-74 (1997)
PMID: 9253417

Function and Biology Details

Reactions catalysed: 
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate.
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. Inother picornavirus reactions Glu may be substituted for Gln, and Ser orThr for Gly.
Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein.
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate+ phosphate + H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136706 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
P1/MAHONEY POLIOVIRUS Chain: 0
Molecule details ›
Chain: 0
Length: 5 amino acids
Theoretical weight: 437 Da
Source organism: Human poliovirus 1 Mahoney
Expression system: Not provided
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 302 amino acids
Theoretical weight: 33.48 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Not provided
UniProt:
  • Canonical: P03300 (Residues: 580-881; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: 2
Capsid protein VP3 Chain: 3
Capsid protein VP4 Chain: 4

Ligands and Environments

2 bound ligands:
Ligand SPH 1 x SPH
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ELLIOTT GX-13
Spacegroup: P21212
Unit cell:
a: 319.95Å b: 355.1Å c: 377.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 not available
Expression system: Not provided

Quick links

Citations

3 review citations

An overview of the evolution of enterovirus 71 and its clinical and public health significance.
McMinn PC. (2002)
2 more

1 mention without citation

Enhanced cellular receptor usage by a bioselected variant of coxsackievirus a21.
Johansson et al. (2004)