› 1atj

X-ray diffraction
2.15Å resolution

RECOMBINANT HORSERADISH PEROXIDASE C1A

Released:
DOI: 10.2210/pdb1atj/pdb
PDB entry 1atj coloured by chain, front view.
PDB entry 1atj coloured by chain, side view.
PDB entry 1atj coloured by chain, top view.
Source organism: Armoracia rusticana
Primary publication:
Crystal structure of horseradish peroxidase C at 2.15 A resolution.
Gajhede M, Schuller DJ, Henriksen A, Smith AT, Poulos TL
Nat Struct Biol 4 1032-8 (1997)
PMID: 9406554

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxidase C1A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 306 amino acids
Theoretical weight: 33.75 KDa
Source organism: Armoracia rusticana
Expression system: Escherichia coli
UniProt:
  • Canonical: P00433 (Residues: 31-336; Coverage: 95%)
Gene names: HPRC1, PRXC1A
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 6 x HEM
1 bound ligand:
Ligand CA 12 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3112
Unit cell:
a: 159.17Å b: 159.17Å c: 114.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.242
Expression system: Escherichia coli

Quick links

Citations

36 review citations

A large family of class III plant peroxidases.
Hiraga et al. (2001)
35 more

35 mentions without citation

Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding.
Krishnamurthy et al. (2008)
34 more