› 1aue

X-ray diffraction
2.33Å resolution

FKBP-RAPAMYCIN BINDING DOMAIN (FRB) OF THE FKBP-RAPAMYCIN ASSOCIATED PROTEIN

Released:
DOI: 10.2210/pdb1aue/pdb
PDB entry 1aue coloured by chain, front view.
PDB entry 1aue coloured by chain, side view.
PDB entry 1aue coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Peptidomimicry by the Effector Element of Rapamycin
Odagaki Y, Clardy J
J. Am. Chem. Soc. 119 10253-10254 (1997)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase mTOR Chains: A, B
Molecule details ›
Chains: A, B
Length: 100 amino acids
Theoretical weight: 12.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42345 (Residues: 2015-2114; Coverage: 4%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains: FKBP12-rapamycin binding domain
Structure domains: FKBP12-rapamycin binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 29.94Å b: 59.21Å c: 123.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.339
Expression system: Escherichia coli BL21

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Citations

3 mentions without citation

Insufficiently dehydrated hydrogen bonds as determinants of protein interactions.
Fernández et al. (2003)
2 more