› 1b00

X-ray diffraction
1.88Å resolution

PHOB RECEIVER DOMAIN FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1b00/pdb
PDB entry 1b00 coloured by chain, front view.
PDB entry 1b00 coloured by chain, side view.
PDB entry 1b00 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Three-dimensional crystal structure of the transcription factor PhoB receiver domain.
Solá M, Gomis-Rüth FX, Serrano L, González A, Coll M
J Mol Biol 285 675-87 (1999)
PMID: 9878437

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate regulon transcriptional regulatory protein PhoB Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AFJ5 (Residues: 1-127; Coverage: 56%)
Gene names: JW0389, b0399, phoB
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 34.11Å b: 60.42Å c: 119.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.28
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Two-component signal transduction.
Stock et al. (2000)
7 more

20 mentions without citation

Bacterial response regulators: versatile regulatory strategies from common domains.
Gao et al. (2007)
19 more