› 1b01

X-ray diffraction
2.56Å resolution

TRANSCRIPTIONAL REPRESSOR COPG/DNA COMPLEX

Released:
DOI: 10.2210/pdb1b01/pdb
PDB entry 1b01 coloured by chain, front view.
PDB entry 1b01 coloured by chain, side view.
PDB entry 1b01 coloured by chain, top view.
Source organism: Streptococcus agalactiae
Primary publication:
The structure of plasmid-encoded transcriptional repressor CopG unliganded and bound to its operator.
Gomis-Rüth FX, Solá M, Acebo P, Párraga A, Guasch A, Eritja R, González A, Espinosa M, del Solar G, Coll M
EMBO J 17 7404-15 (1998)
PMID: 9857196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113454 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein CopG Chains: A, B
Molecule details ›
Chains: A, B
Length: 45 amino acids
Theoretical weight: 5.12 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13920 (Residues: 1-45; Coverage: 100%)
Gene names: copG, repA
Sequence domains: Ribbon-helix-helix protein, copG family
Structure domains: Met repressor-like
DNA (5'-D(*CP*CP*CP*GP*TP*GP*CP*AP*CP*TP*CP*AP*AP*TP*GP*CP*AP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.75 KDa
Source organism: Streptococcus agalactiae
Expression system: Not provided
DNA (5'-D(*GP*AP*TP*TP*GP*CP*AP*TP*TP*GP*AP*GP*TP*GP*CP*AP*CP*GP*G)-3') Chain: F
Molecule details ›
Chain: F
Length: 19 nucleotides
Theoretical weight: 5.9 KDa
Source organism: Streptococcus agalactiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P4322
Unit cell:
a: 40.3Å b: 40.3Å c: 221.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.316
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
16 more

17 mentions without citation

Molecular Evolution of Transition Metal Bioavailability at the Host-Pathogen Interface.
Antelo et al. (2021)
16 more