› 1b12

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF TYPE 1 SIGNAL PEPTIDASE FROM ESCHERICHIA COLI IN COMPLEX WITH A BETA-LACTAM INHIBITOR

Released:
DOI: 10.2210/pdb1b12/pdb
PDB entry 1b12 coloured by chain, front view.
PDB entry 1b12 coloured by chain, side view.
PDB entry 1b12 coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor.
Paetzel M, Dalbey RE, Strynadka NC
Nature 396 186-90 (1998)
PMID: 9823901

Function and Biology Details

Reaction catalysed: 
Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal peptidase I Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 27.84 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00803 (Residues: 77-324; Coverage: 77%)
Gene names: JW2552, b2568, lepB
Sequence domains: Signal peptidase, peptidase S26
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 1PN 4 x 1PN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 110.7Å b: 113.2Å c: 99.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Regulated intramembrane proteolysis: a control mechanism conserved from bacteria to humans.
Brown et al. (2000)
39 more

29 mentions without citation

Unconventional serine proteases: variations on the catalytic Ser/His/Asp triad configuration.
Ekici et al. (2008)
28 more