› 1b23

X-ray diffraction
2.6Å resolution

E. coli cysteinyl-tRNA and T. aquaticus elongation factor EF-TU:GTP ternary complex

Released:
DOI: 10.2210/pdb1b23/pdb
PDB entry 1b23 coloured by chain, front view.
PDB entry 1b23 coloured by chain, side view.
PDB entry 1b23 coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of Cys-tRNACys-EF-Tu-GDPNP reveals general and specific features in the ternary complex and in tRNA.
Nissen P, Thirup S, Kjeldgaard M, Nyborg J
Structure 7 143-56 (1999)
PMID: 10368282

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169612 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Elongation factor Tu Chain: P
Molecule details ›
Chain: P
Length: 405 amino acids
Theoretical weight: 44.74 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01698 (Residues: 2-406; Coverage: 100%)
Gene names: tuf, tufA
Sequence domains:
Structure domains:
CYSTEINYL TRNA Chain: R
Molecule details ›
Chain: R
Length: 74 nucleotides
Theoretical weight: 23.93 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand CYS 1 x CYS
Ligand SO4 2 x SO4
Ligand GNP 1 x GNP
5 modified residues:
Ligand 4SU 1 x 4SU
Ligand H2U 2 x H2U
Ligand PSU 3 x PSU
Ligand MIA 1 x MIA
Ligand 5MU 1 x 5MU

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: F222
Unit cell:
a: 126.75Å b: 132.98Å c: 154.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.263
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

23 review citations

Elongation factors in protein biosynthesis.
Andersen et al. (2003)
22 more

49 mentions without citation

Mechanism of protein biosynthesis in mammalian mitochondria.
Christian et al. (2012)
48 more