› 1b2l

X-ray diffraction
1.6Å resolution

ALCOHOL DEHYDROGENASE FROM DROSOPHILA LEBANONENSIS: TERNARY COMPLEX WITH NAD-CYCLOHEXANONE

Released:
DOI: 10.2210/pdb1b2l/pdb
PDB entry 1b2l coloured by chain, front view.
PDB entry 1b2l coloured by chain, side view.
PDB entry 1b2l coloured by chain, top view.
Source organism: Scaptodrosophila lebanonensis
Primary publication:
The catalytic reaction and inhibition mechanism of Drosophila alcohol dehydrogenase: observation of an enzyme-bound NAD-ketone adduct at 1.4 A resolution by X-ray crystallography.
Benach J, Atrian S, Gonzàlez-Duarte R, Ladenstein R
J Mol Biol 289 335-55 (1999)
PMID: 10366509

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 27.82 KDa
Source organism: Scaptodrosophila lebanonensis
UniProt:
  • Canonical: P10807 (Residues: 1-254; Coverage: 100%)
Gene name: Adh
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDC 1 x NDC
3 bound ligands:
Ligand CA 1 x CA
Ligand CYH 1 x CYH
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P3221
Unit cell:
a: 54.1Å b: 54.1Å c: 168.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.212

Quick links

Citations

6 review citations

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
5 more

5 mentions without citation

The molecular aspects of absorption and metabolism of carotenoids and retinoids in vertebrates.
Widjaja-Adhi et al. (2020)
4 more