› 1b2y

X-ray diffraction
3.2Å resolution

STRUCTURE OF HUMAN PANCREATIC ALPHA-AMYLASE IN COMPLEX WITH THE CARBOHYDRATE INHIBITOR ACARBOSE

Released:
DOI: 10.2210/pdb1b2y/pdb
PDB entry 1b2y coloured by chain, front view.
PDB entry 1b2y coloured by chain, side view.
PDB entry 1b2y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human pancreatic alpha-amylase in complex with carbohydrate and proteinaceous inhibitors.
Nahoum V, Roux G, Anton V, Rougé P, Puigserver A, Bischoff H, Henrissat B, Payan F
Biochem J 346 Pt 1 201-8 (2000)
PMID: 10657258

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Pancreatic alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 55.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P04746 (Residues: 16-511; Coverage: 100%)
Gene name: AMY2A
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, AC1, GLC, G6D
Ligand BGC 1 x BGC
Ligand AC1 1 x AC1
Ligand GLC 1 x GLC
Ligand G6D 1 x G6D
2 bound ligands:
Ligand CL 1 x CL
Ligand CA 1 x CA
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 53.11Å b: 75.1Å c: 137.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.217

Quick links

Citations

11 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
10 more

41 mentions without citation

Bioactive oligosaccharide natural products.
McCranie et al. (2014)
40 more