› 1b3t

X-ray diffraction
2.2Å resolution

EBNA-1 NUCLEAR PROTEIN/DNA COMPLEX

Released:
DOI: 10.2210/pdb1b3t/pdb
Model geometry
Fit model/data
PDB entry 1b3t coloured by chain, front view.
PDB entry 1b3t coloured by chain, side view.
PDB entry 1b3t coloured by chain, top view.
Source organism: human gammaherpesvirus 4
Primary publication:
The 2.2 A structure of a permanganate-sensitive DNA site bound by the Epstein-Barr virus origin binding protein, EBNA1.
Bochkarev A, Bochkareva E, Frappier L, Edwards AM
J Mol Biol 284 1273-8 (1998)
PMID: 9878348

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113457 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Epstein-Barr nuclear antigen 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.01 KDa
Source organism: Human gammaherpesvirus 4
Expression system: Escherichia coli
UniProt:
  • Canonical: P03211 (Residues: 461-607; Coverage: 23%)
Gene names: BKRF1, EBNA1
Sequence domains: Epstein Barr virus nuclear antigen-1, DNA-binding domain
Structure domains: Epstein Barr virus nuclear antigen-1, DNA-binding domain
DNA (5'-D(*GP*GP*GP*AP*AP*GP*CP*AP*TP*AP*TP*GP*CP*TP*TP*CP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: human gammaherpesvirus 4
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 60.083Å b: 64.501Å c: 111.938Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
4 more

32 mentions without citation

Control of Viral Latency by Episome Maintenance Proteins.
De Leo et al. (2020)
31 more