› 1b6b

X-ray diffraction
2.5Å resolution

MELATONIN BIOSYNTHESIS: THE STRUCTURE OF SEROTONIN N-ACETYLTRANSFERASE AT 2.5 A RESOLUTION SUGGESTS A CATALYTIC MECHANISM

Released:
DOI: 10.2210/pdb1b6b/pdb
PDB entry 1b6b coloured by chain, front view.
PDB entry 1b6b coloured by chain, side view.
PDB entry 1b6b coloured by chain, top view.
Source organism: Ovis aries
Primary publication:
Melatonin biosynthesis: the structure of serotonin N-acetyltransferase at 2.5 A resolution suggests a catalytic mechanism.
Hickman AB, Klein DC, Dyda F
Mol Cell 3 23-32 (1999)
PMID: 10024876

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serotonin N-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 19.56 KDa
Source organism: Ovis aries
Expression system: Escherichia coli
UniProt:
  • Canonical: Q29495 (Residues: 28-201; Coverage: 84%)
Gene names: AANAT, SNAT
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P61
Unit cell:
a: 56.61Å b: 56.61Å c: 207.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.282
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Histone acetyltransferases.
Roth et al. (2001)
17 more

10 mentions without citation

Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).
Salah Ud-Din et al. (2016)
9 more