› 1b6v

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF A HYBRID BETWEEN RIBONUCLEASE A AND BOVINE SEMINAL RIBONUCLEASE

Released:
DOI: 10.2210/pdb1b6v/pdb
PDB entry 1b6v coloured by chain, front view.
PDB entry 1b6v coloured by chain, side view.
PDB entry 1b6v coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of a hybrid between ribonuclease A and bovine seminal ribonuclease--the basic surface, at 2.0 A resolution.
Vatzaki EH, Allen SC, Leonidas DD, Trautwein-Fritz K, Stackhouse J, Benner SA, Acharya KR
Eur J Biochem 260 176-82 (1999)
PMID: 10091597

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.73 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 29.02Å b: 75.66Å c: 53.93Å
α: 90° β: 101.15° γ: 90°
R-values:
R R work R free
0.182 0.182 0.256
Expression system: Escherichia coli

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Citations

3 review citations

Ribonucleases and their antitumor activity.
Matousek J. (2001)
2 more