› 1b7e

X-ray diffraction
2.9Å resolution

TRANSPOSASE INHIBITOR

Released:
DOI: 10.2210/pdb1b7e/pdb
PDB entry 1b7e coloured by chain, front view.
PDB entry 1b7e coloured by chain, side view.
PDB entry 1b7e coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution.
Davies DR, Mahnke Braam L, Reznikoff WS, Rayment I
J Biol Chem 274 11904-13 (1999)
PMID: 10207011

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175136 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transposase for transposon Tn5 Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46731 (Residues: 56-476; Coverage: 88%)
Gene names: tnp, tnpA
Sequence domains: Transposase DDE domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TPT 2 x TPT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 181.8Å b: 71.9Å c: 41.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 not available
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The RAG proteins and V(D)J recombination: complexes, ends, and transposition.
Fugmann et al. (2000)
11 more

3 mentions without citation

DDE transposases: Structural similarity and diversity.
Nesmelova et al. (2010)
2 more