› 1b8n

X-ray diffraction
2Å resolution

PURINE NUCLEOSIDE PHOSPHORYLASE

Released:
DOI: 10.2210/pdb1b8n/pdb
PDB entry 1b8n coloured by chain, front view.
PDB entry 1b8n coloured by chain, side view.
PDB entry 1b8n coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Atomic dissection of the hydrogen bond network for transition-state analogue binding to purine nucleoside phosphorylase.
Kicska GA, Tyler PC, Evans GB, Furneaux RH, Shi W, Fedorov A, Lewandowicz A, Cahill SM, Almo SC, Schramm VL
Biochemistry 41 14489-98 (2002)
PMID: 12463747

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-157332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 31.69 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P55859 (Residues: 1-284; Coverage: 98%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand PO4 1 x PO4
Ligand IMG 1 x IMG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P213
Unit cell:
a: 90.32Å b: 90.32Å c: 90.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 0.246

Quick links

Citations

11 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
10 more

1 mention without citation

istar: a web platform for large-scale protein-ligand docking.
Li et al. (2014)