› 1b8z

X-ray diffraction
1.6Å resolution

HU FROM THERMOTOGA MARITIMA

Released:
DOI: 10.2210/pdb1b8z/pdb
PDB entry 1b8z coloured by chain, front view.
PDB entry 1b8z coloured by chain, side view.
PDB entry 1b8z coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima.
Christodoulou E, Vorgias CE
Acta Crystallogr D Biol Crystallogr 54 1043-5 (1998)
PMID: 9757133

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein HU Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 10.02 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: P36206 (Residues: 1-90; Coverage: 100%)
Gene names: TM_0266, hup
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P43
Unit cell:
a: 46.12Å b: 46.12Å c: 77.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Nucleoid-Associated Protein HU: A Lilliputian in Gene Regulation of Bacterial Virulence.
Stojkova et al. (2019)
6 other articles

8 mentions without citation

Flexible DNA bending in HU-DNA cocrystal structures.
Swinger et al. (2003)
7 more