› 1b9c

X-ray diffraction
2.4Å resolution

Green Fluorescent Protein Mutant F99S, M153T and V163A

Released:
DOI: 10.2210/pdb1b9c/pdb
PDB entry 1b9c coloured by chain, front view.
PDB entry 1b9c coloured by chain, side view.
PDB entry 1b9c coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Crystal structure and refolding properties of the mutant F99S/M153T/V163A of the green fluorescent protein.
Battistutta R, Negro A, Zanotti G
Proteins 41 429-37 (2000)
PMID: 11056031

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 26.74 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 4 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 83.48Å b: 85.37Å c: 140.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The folding, stability and conformational dynamics of beta-barrel fluorescent proteins.
Hsu et al. (2009)
5 more

11 mentions without citation

Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes.
Stepanenko et al. (2008)
10 more