› 1bdf

X-ray diffraction
2.5Å resolution

STRUCTURE OF ESCHERICHIA COLI RNA POLYMERASE ALPHA SUBUNIT N-TERMINAL DOMAIN

Released:
DOI: 10.2210/pdb1bdf/pdb
PDB entry 1bdf coloured by chain, front view.
PDB entry 1bdf coloured by chain, side view.
PDB entry 1bdf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain.
Zhang G, Darst SA
Science 281 262-6 (1998)
PMID: 9657722

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 25.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-235; Coverage: 71%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: R32
Unit cell:
a: 117.2Å b: 117.2Å c: 350.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.308
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Transcription activation by catabolite activator protein (CAP).
Busby et al. (1999)
13 more

12 mentions without citation

Catabolite activator protein: DNA binding and transcription activation.
Lawson et al. (2004)
11 more