› 1bg3

X-ray diffraction
2.8Å resolution

RAT BRAIN HEXOKINASE TYPE I COMPLEX WITH GLUCOSE AND INHIBITOR GLUCOSE-6-PHOSPHATE

Released:
DOI: 10.2210/pdb1bg3/pdb
PDB entry 1bg3 coloured by chain, front view.
PDB entry 1bg3 coloured by chain, side view.
PDB entry 1bg3 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The structure of mammalian hexokinase-1.
Mulichak AM, Wilson JE, Padmanabhan K, Garavito RM
Nat Struct Biol 5 555-60 (1998)
PMID: 9665168

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 918 amino acids
Theoretical weight: 102.55 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P05708 (Residues: 1-918; Coverage: 100%)
Gene name: Hk1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BGC 4 x BGC
Ligand G6P 4 x G6P
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 132.1Å b: 77.1Å c: 137.1Å
α: 90° β: 96° γ: 90°
R-values:
R R work R free
0.196 0.196 0.251

Quick links

Citations

6 review citations

Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family.
Kawai et al. (2005)
5 more

5 mentions without citation

Structural studies of ROK fructokinase YdhR from Bacillus subtilis: insights into substrate binding and fructose specificity.
Nocek et al. (2011)
4 more