› 1bg9

X-ray diffraction
2.8Å resolution

BARLEY ALPHA-AMYLASE WITH SUBSTRATE ANALOGUE ACARBOSE

Released:
DOI: 10.2210/pdb1bg9/pdb
PDB entry 1bg9 coloured by chain, front view.
PDB entry 1bg9 coloured by chain, side view.
PDB entry 1bg9 coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Molecular structure of a barley alpha-amylase-inhibitor complex: implications for starch binding and catalysis.
Kadziola A, Søgaard M, Svensson B, Haser R
J Mol Biol 278 205-17 (1998)
PMID: 9571044

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-amylase type B isozyme Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.99 KDa
Source organism: Hordeum vulgare
UniProt:
  • Canonical: P04063 (Residues: 25-427; Coverage: 100%)
Gene name: AMY1.2
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, DAF
Ligand BGC 1 x BGC
Ligand DAF 1 x DAF
2 bound ligands:
Ligand CA 3 x CA
Ligand AF1 1 x AF1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3121
Unit cell:
a: 135.2Å b: 135.2Å c: 79.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.151 0.249

Quick links

Citations

12 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
11 more

4 mentions without citation

Chapter 4. Predicting and characterizing protein functions through matching geometric and evolutionary patterns of binding surfaces.
Liang et al. (2008)
3 more