› 1bj1

X-ray diffraction
2.4Å resolution

VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH A NEUTRALIZING ANTIBODY

Released:
DOI: 10.2210/pdb1bj1/pdb
PDB entry 1bj1 coloured by chain, front view.
PDB entry 1bj1 coloured by chain, side view.
PDB entry 1bj1 coloured by chain, top view.
Source organisms:
Primary publication:
VEGF and the Fab fragment of a humanized neutralizing antibody: crystal structure of the complex at 2.4 A resolution and mutational analysis of the interface.
Muller YA, Chen Y, Christinger HW, Li B, Cunningham BC, Lowman HB, de Vos AM
Structure 6 1153-67 (1998)
PMID: 9753694

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225488 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab fragment, light chain Chains: J, L
Molecule details ›
Chains: J, L
Length: 214 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab fragment, heavy chain Chains: H, K
Molecule details ›
Chains: H, K
Length: 231 amino acids
Theoretical weight: 24.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
N-VEGF Chains: V, W
Molecule details ›
Chains: V, W
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 214-315; Coverage: 26%)
Gene names: VEGF, VEGFA
Sequence domains: VEGF heparin-binding domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 89.86Å b: 66.98Å c: 140.51Å
α: 90° β: 94.27° γ: 90°
R-values:
R R work R free
0.196 0.196 0.266
Expression system: Escherichia coli

Quick links

Citations

36 review citations

Discovery and development of bevacizumab, an anti-VEGF antibody for treating cancer.
Ferrara et al. (2004)
35 more

73 mentions without citation

Tying the knot: the cystine signature and molecular-recognition processes of the vascular endothelial growth factor family of angiogenic cytokines.
Iyer et al. (2011)
72 more