› 1bk7

X-ray diffraction
1.75Å resolution

RIBONUCLEASE MC1 FROM THE SEEDS OF BITTER GOURD

Released:
DOI: 10.2210/pdb1bk7/pdb
Model geometry
Fit model/data
PDB entry 1bk7 coloured by chain, front view.
PDB entry 1bk7 coloured by chain, side view.
PDB entry 1bk7 coloured by chain, top view.
Source organism: Momordica charantia
Primary publication:
Crystal structure of a ribonuclease from the seeds of bitter gourd (Momordica charantia) at 1.75 A resolution.
Nakagawa A, Tanaka I, Sakai R, Nakashima T, Funatsu G, Kimura M
Biochim Biophys Acta 1433 253-60 (1999)
PMID: 10446375

Function and Biology Details

Reaction catalysed: 
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease MC Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.23 KDa
Source organism: Momordica charantia
UniProt:
  • Canonical: P23540 (Residues: 1-191; Coverage: 100%)
Gene name: MC1
Sequence domains: Ribonuclease T2 family
Structure domains: Ribonuclease T2-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MACSCIENCE M18X
Spacegroup: P212121
Unit cell:
a: 38.8Å b: 67.97Å c: 75.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.241

Quick links

Citations

1 review citation

Mutational origin of new mating type specificities in flowering plants.
Uyenoyama MK. (2000)
15 other articles

6 mentions without citation

One- and two-body decomposable Poisson-Boltzmann methods for protein design calculations.
Marshall et al. (2005)
5 more