› 1bl0

X-ray diffraction
2.3Å resolution

MULTIPLE ANTIBIOTIC RESISTANCE PROTEIN (MARA)/DNA COMPLEX

Released:
DOI: 10.2210/pdb1bl0/pdb
PDB entry 1bl0 coloured by chain, front view.
PDB entry 1bl0 coloured by chain, side view.
PDB entry 1bl0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator.
Rhee S, Martin RG, Rosner JL, Davies DR
Proc Natl Acad Sci U S A 95 10413-8 (1998)
PMID: 9724717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113494 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Multiple antibiotic resistance protein MarA Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 15.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACH5 (Residues: 1-127; Coverage: 100%)
Gene names: JW5249, b1531, marA
Sequence domains: Helix-turn-helix domain
Structure domains: Homeodomain-like
DNA (5'-D(*GP*GP*GP*GP*AP*TP*TP*TP*AP*GP*CP*AP*AP*AP*AP*CP*GP*TP*GP*GP*CP*AP* TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 24 nucleotides
Theoretical weight: 7.46 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*CP*CP*GP*AP*TP*GP*CP*CP*AP*CP*GP*TP*TP*TP*TP*GP*CP*TP*AP*AP*AP*TP* CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.28 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P41212
Unit cell:
a: 47.1Å b: 47.1Å c: 298.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.303
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
24 more

53 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
52 more