› 1bn7

X-ray diffraction
1.5Å resolution

HALOALKANE DEHALOGENASE FROM A RHODOCOCCUS SPECIES

Released:
DOI: 10.2210/pdb1bn7/pdb
PDB entry 1bn7 coloured by chain, front view.
PDB entry 1bn7 coloured by chain, side view.
PDB entry 1bn7 coloured by chain, top view.
Source organism: Rhodococcus sp.
Primary publication:
Haloalkane dehalogenases: structure of a Rhodococcus enzyme.
Newman J, Peat TS, Richard R, Kan L, Swanson PE, Affholter JA, Holmes IH, Schindler JF, Unkefer CJ, Terwilliger TC
Biochemistry 38 16105-14 (1999)
PMID: 10587433

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.37 KDa
Source organism: Rhodococcus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P59336 (Residues: 1-294; Coverage: 100%)
Gene name: dhaA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21212
Unit cell:
a: 93.63Å b: 79.89Å c: 42.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.178
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Biodegradation and Rhodococcus--masters of catabolic versatility.
Larkin et al. (2005)
12 more

16 mentions without citation

Maximum-likelihood density modification.
Terwilliger TC. (2000)
15 more