› 1bo4

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF A GCN5-RELATED N-ACETYLTRANSFERASE: SERRATIA MARESCENS AMINOGLYCOSIDE 3-N-ACETYLTRANSFERASE

Released:
DOI: 10.2210/pdb1bo4/pdb
PDB entry 1bo4 coloured by chain, front view.
PDB entry 1bo4 coloured by chain, side view.
PDB entry 1bo4 coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase.
Wolf E, Vassilev A, Makino Y, Sali A, Nakatani Y, Burley SK
Cell 94 439-49 (1998)
PMID: 9727487

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 18.49 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53396 (Residues: 1-168; Coverage: 95%)
Gene name: aacC1
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand SPD 1 x SPD
Ligand COA 2 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2
Unit cell:
a: 78.54Å b: 102.26Å c: 50.14Å
α: 90° β: 93.73° γ: 90°
R-values:
R R work R free
0.201 0.201 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

31 review citations

Comparative protein structure modeling of genes and genomes.
Martí-Renom et al. (2000)
30 more

13 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
12 more