› 1bof

X-ray diffraction
2.2Å resolution

GI-ALPHA-1 BOUND TO GDP AND MAGNESIUM

Released:
DOI: 10.2210/pdb1bof/pdb
PDB entry 1bof coloured by chain, front view.
PDB entry 1bof coloured by chain, side view.
PDB entry 1bof coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structures of the G protein Gi alpha 1 complexed with GDP and Mg2+: a crystallographic titration experiment.
Coleman DE, Sprang SR
Biochemistry 37 14376-85 (1998)
PMID: 9772163

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.27 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 2-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I4
Unit cell:
a: 121.747Å b: 121.747Å c: 68.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.268
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural features of the G-protein/GPCR interactions.
Moreira IS. (2014)
4 more

17 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
16 more